Benefits of Hybridoma Sequencing by NGS

Digital Proteomics has developed a method for deep sequencing of hybridomas built on our bulk antibody repertoire technology ReptorTM. The benefits of our process include:

  • Economy of scale – Multiplexed next-generation sequencing and automated data processing makes sequencing many hybridomas cost-effective.
  • Accurate detection of minor clones – Deep sequencing each hybridoma reveals contaminating, minor clones that confound other sequencing methods.
  • Stronger IP protection – Sequencing all hybridomas from your campaign reveals related sequences that can contribute to a stronger patent application.
  • Security – Knowing the sequence of your valuable hybridomas gives you peace of mind in the event of hybridoma loss.
  • Low sample requirements – The sensitivity of next-generation sequencing enables our process to start from as few as 10,000 cells.

Reptor Hybridoma Sequencing Process

Sequencing by ReptorTM is available for

  • mouse (all isotypes)
  • human (all isotypes)
  • rabbit (IgG/K)

Interested in sequencing hybridomas from a different species? Contact us to discuss how our process could work for your project.

Material Requirements – At least 10,000 cells per hybridoma.

Turn around time – 3 weeks

Contact us today for more info!

Reptor Hybridoma Process

Hybridoma Sequencing Deliverables

  • Dominant and minor sequences for each hybridoma

  • Automated CDR detection on each sequence

  • Clonality assessment for each hybridoma

Related Services

Digital Proteomics offers a suite of antibody sequencing and discovery services to complement your hybridoma sequencing workflow.

  • Proteomic mAb verification – We confirm by mass spectrometry that the produced antibody matches the hybridoma sequence(s).
  • ReptorTM bulk B-cell receptor sequencing – Complement your hybridoma campaign by sequencing related members of your hybridoma from remaining lymph nodes, spleen, or blood.
  • ValensTM de novo protein sequencing – We determine the full-length sequence of your antibody directly from the protein, no hybridoma needed.

Did you know many hybridomas are not monoclonal? We can determine clonality of your hybridoma with our process. Read more about a recent assessment of 185 hybridomas in When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions.