Protein Sequence Confirmation with Valens

Do you want to confirm the sequence of your protein or antibody sample? We offer sequence confirmation by mass spectrometry using ValensTM. We’ve successfully confirmed sequences for the following applications:

  • Hybridoma verification – Confirm the secreted antibody matches the expected genomic sequence.
  • Truncation detection – Identify the translation boundaries and post-translational processing events of your sequence.
  • Recombinant protein confirmation – Assess how well the expressed protein matches the intended gene sequence.

Protein Sequence Confirmation Process

Sequence confirmation is a distinct process from de novo protein sequencing. In sequence confirmation, we have a putative sequence against which to verify the sample. We provide a probability of each amino acid being correct.

Material Requirements – 100ug of 95% pure protein.

Turn around time – 4 weeks

Work performed 100% in the USA.

Don’t have a putative sequence? You will need de novo protein sequencing and we can help!

Contact us today for more info!

Protein Sequence Confirmation Process

Sequence Confirmation Deliverables

  • Interactive report with presentation ready images

  • Peptide coverage of putative protein

  • Per residue confidence score

Related Services

Digital Proteomics offers a suite of antibody sequencing and discovery services to complement your protein sequence confirmation workflow.

ReptorTM hybridoma sequencing – We provide economical, high-throughput hybridoma sequencing using next-generation sequencing.

ReptorTM bulk B-cell receptor sequencing – Complement your hybridoma campaign by sequencing related members of your hybridoma from remaining lymph nodes, spleen, or blood.

ValensTM de novo protein sequencing – We determine the full-length sequence of your antibody directly from the protein, no hybridoma needed.

Is your hybridoma monoclonal? Our process can determine the sequence of your secreted protein and confirm it matches to your hybridoma sequence.