Why single domain?
Single domain antibodies contain only heavy chains and are devoid of light chains. They have unique properties of stability and solubility, making them attractive for harsh environments such as the tumor microenvironment. Their structure also enables these antibodies to recognize epitopes that are inaccessible to conventional antibodies. We recently analyzed a naive llama repertoire and cover a little more of the biology of single domain antibodies in a blog post.
From which camelids do you generate single domain antibodies?
We can discover antibodies from both llamas and alpacas. Which animal to use is a matter of client preference. We are able to extend Alicanto® to other camelids upon request. If you are interested in single domain antibodies from other species, we are eager to collaborate!
What are the material requirements?
We require 2.5 mg of antigen for immunization, affinity purification, and ELISA. The affinity purification can be customized to target specific variants, sub-domains, or cross-reactive species which would require additional antigen.
Can I send you samples from my immunization?
In some cases, we may be able to work with an immunization in progress, or a completed immunization.Contact us today to discuss the particulars of your projects!